6-Hydroxykaempferol 3,6-diglucoside

Natural Med., 2005, 59(5):241-4.

Antiplatelet Aggregatory Effects of the Constituents Isolated from the Flower of Cartham.ua tinctorius[Reference: WebLink]

METHODS AND RESULTS:
Four constituents, 6-hydroxykaempferol 3,6,7-tri-O-glucoside (1), 6-hydroxykaempferol 3-O-rutinoside-6-O-glucoside (2), 6-hydroxykaempferol 3,6-di-O-glucoside (6-Hydroxykaempferol 3,6-diglucoside,3), and syringin (4) were isolated from the flower of Carthamus tinctorius L. and were examined for the inhibitory effects on platelet aggregation. The platelet aggregation was monitored by a platelet aggregometer employing a laser-scattering method. The compounds were identified on the basis of NMR spectral data.
CONCLUSIONS:
Compound 1, 3 and 4 inhibited platelet aggregation induced by collagen. Compound 4 also showed an antiplatelet aggregatory effect when the platelet aggregation was induced by adenosine 5'-diphosphate (ADP). However, 2 showed no inhibitory effect on platelet aggregation induced by collagen or ADP. Furthermore, 1 and 3 showed weak inhibitory effects on the ADP-induced platelet aggregation.

Biomed Chromatogr. 2006 Aug;20(8):821-6.

An HPLC/MS method for identifying major constituents in the hypocholesterolemic extracts of Chinese medicine formula 'Xue-Fu-Zhu-Yu decoction'.[Pubmed: 16345012]

METHODS AND RESULTS:
In this paper, HPLC-ESI-MS technique was used to analyze the hypocholesterolemic extracts of 'Xue-Fu-Zhu-Yu decoction', a traditional Chinese medicine consisting of six crude drugs (i.e. Paeonia lactiflora, Ligusticum chuanxiong, Citrus aurantium, Carthamus tinctorius, Prunus persica and Bupleurum falcatum). A total of 17 compounds were identified and their plant derivations were also distinguished. Nine compounds among them were unambiguously determined as oxypaeoniflorin, amygdalin, albiflorin, paeoniflorin, ferulic acid, naringin, hesperidin, senkyunolide I and neohesperidin by comparing the retention times (tR), UV spectra and m/z values with those of the reference compounds.
CONCLUSIONS:
The other eight compounds were tentatively identified as prunasin, 6-Hydroxykaempferol 3,6-diglucoside, 6-hydroxykaempferol 3-rutinoside-6-glucoside, galloylpaeoniflorin, 6-hydroxy-kaempferol 3-glucoside, anhydrosafflor yellow B, narirutin and kaempferol 3-rutinoside by MS2 spectra and the comparison of their UV spectra and MS spectra with literature data.