4'-O-Glucosylvitexin
Reference standards.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Journal of Liquid Chromatography & Related Technologies,2008,32(15):2216-2231.
Isolation and Purification of Three Flavonoids from the Hawthorn Leaves by High Speed Countercurrent Chromatography, Combined with Isocratic Preparative Reversed-Phase High Performance Liquid Chromatography.[Reference:
WebLink]
METHODS AND RESULTS:
Three flavonoids including 4″′-O-rhamnosylrutin, 2″-O-rhamnosylvitexin, and 4″-O-glucosylvitexin were isolated and purified from the hawthorn leaves by high speed countercurrent chromatography (HSCCC), combined together with isocratic preparative reversed-phase high performance liquid chromatography.
Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of n-butanol-water (1:1, v/v). After two useful fractions were obtained by HSCCC using the optimal solvent system, one of them, fraction II, was further purified by reversed-phase semipreparative high performance liquid chromatography.
CONCLUSIONS:
From 500 mg partially purified extract of the hawthorn leaves, 188.4 mg 2″-O-rhamnosylvitexin, 28.5 mg 4″′-O-rhamnosylrutin, and 55.3 mg 4″-O-glucosylvitexin with purities of over 97%, respectively, were obtained. The structure identification of all pure fractions was carried out by UV, MS, 1H NMR and 13C NMR.
