4-Bromomethyl-7-methoxycoumarin
4-Bromomethyl-7-methoxycoumarin as a new fluorescence label for fatty acids.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Journal of Chromatography A, 1978,158(1):207-214.
Labeling of fatty acids with 4-bromomethyl-7-methoxycoumarin via crown ether catalyst for fluorimetric detection in high-performance liquid chromatography.[Reference:
WebLink]
METHODS AND RESULTS:
A method for the fluorescence labeling of fatty acids with 4-Bromomethyl-7-methoxycoumarin using 18-crown-6 as a catalyst is described. The procedure is rapid, simple and requires little or no experience in derivatization techniques. The amounts of the derivatives are linearly related to the amount of the starting fatty acids and the procedure can therefore be used in the quantitative analysis of these solutes.
CONCLUSIONS:
Using reversed-phase chromatography, detection levels as low as 9 pmole could be realized. The separations of several derivatives using a reversed-phase system are discussed.
