2H-1-Benzopyran-7-yloxy
Reference standards.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Toxicological & Environmental Chemistry Reviews, 2009, 91(5):999-1003.
Antimicrobial and cytotoxic activity of the stem and root bark extracts of Quassia undulata and isolated constituents.[Reference:
WebLink]
METHODS AND RESULTS:
The stem and root barks of Quassia undulata were exhaustively extracted with methanol to give the methanolic extracts: MeQuS and MeQuR, respectively. The chloroform extracts were obtained and fractionated on an open column of silica gel by gradient elution with chloroform and chloroform–methanol mixtures. Purification of fractions afforded the isolation of four compounds, namely: 3′-(8-hydroxy,6-methoxy,2-oxo-2H-1-Benzopyran-7-yloxy)-3′,4′-dihydro, 7′-methoxy,2H-1-benzopyran (eniotorin) (1) a new bisbenzopyran coumarin, 7-hydroxy-6-methoxy-2H-1-benzopyran-2-one (scopoletin) (2) (a coumarin); glaucarubinone (3) and 15-desacetylundulatone (4) (quassinoids). The antimicrobial activities of the extracts and isolated compounds were evaluated against five microbes, namely: Staphylococcus aureus; Eschericha coli; Pseudomonas aeruginosa; Klebsiella pneumonia and Candida albicans.
CONCLUSIONS:
The extracts exhibited a broad-spectrum activity against the tested microbes. Cytotoxic activities of the extracts and compounds isolated from them were also examined against brine shrimps, Artemia salina. The extracts and the compounds showed significant dose-dependent activities.
