Stevia impurity (13-[(2-O-6-deoxy-¦Â-D-glucopyranosyl-3-O-¦Â-D-glucopyranosyl-¦Â-D-glucopyranosyl)oxy]ent-kaur-16-en-19-oic acid ¦Â-D-glucopyranosyl ester)
Reference standards.
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Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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Structures of the novel diterpene glycosides from Stevia rebaudiana.[Reference:
WebLink]
METHODS AND RESULTS:
From the commercial extract of the leaves of Stevia rebaudiana, two new diterpenoid glycosides were isolated besides the known steviol glycosides including stevioside, rebaudiosides A-F, rubusoside, and dulcoside A.
CONCLUSIONS:
The structures of the two new compounds were identified as 13-[(2-O-6-deoxy-β-d-glucopyranosyl-β-d-glucopyranosyl)oxy] ent-kaur-16-en-19-oic acid β-d-glucopyranosyl ester (1), and Stevia impurity (13-[(2-O-6-deoxy-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]ent-kaur-16-en-19-oic acid β-D-glucopyranosyl ester) (2), on the basis of extensive NMR and MS spectral data as well as chemical studies.
![Stevia impurity (13-[(2-O-6-deoxy-¦Â-D-glucopyranosyl-3-O-¦Â-D-glucopyranosyl-¦Â-D-glucopyranosyl)oxy]ent-kaur-16-en-19-oic acid ¦Â-D-glucopyranosyl ester)](http://www.chemfaces.com/structural/Stevia-impurity-1-CFN95229.jpg "Stevia impurity (13-[(2-O-6-deoxy-¦Â-D-glucopyranosyl-3-O-¦Â-D-glucopyranosyl-¦Â-D-glucopyranosyl)oxy]ent-kaur-16-en-19-oic acid ¦Â-D-glucopyranosyl ester)")
