Phorbol
Phorbol is a natural product from Euphorbia pekinensis Rupr.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Chinese Journal of Spectroscopy Laboratory, 2011 , 28 (2) :801-805.
Determination of Phorbol in the Seeds of Croton Tiglium Linn by RP-HPLC[Reference:
WebLink]
METHODS AND RESULTS:
The determination of Phorbol in the seeds of Croton tiglium linn by HPLC was investigated. Shimadzu LC-2010 HPLC,Diamonsil C18 column(250mm×4.6mm,5μm) were uesd,and the mobile phase was consisted of acetonitrile-water(gradient elution) with the flow rate of 1mL/min.The detection wavelength was 234nm,and the column temperature was 25℃.There was a good linear relationship of Phorbol in the range of 6.096—101.600μg/mL(r=0.9999),while the average standard recovery was 96.40% with RSD of 1.03%.
CONCLUSIONS:
The method is established for determination of Phorbol in the seeds of Croton tiglium linn by RP-HPLC,and provides a means for future study.
