Osmundacetone
Reference standards.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Isolation and determination of osmundacetone in Osmundae Rhizoma.[Reference:
WebLink]
To study the quantitative determination method for Osmundae Rhizoma (the rhizome of Osmunda japonica).
METHODS AND RESULTS:
Osmundacetone was isolated from Osmundae Rhizoma with silica gel column chromatography. HPLC was used to determine Osmundacetone content in Osmundae Rhizoma and the optimum condition was chosen. Osmundacetone was used as reference standard. The Kromasil C18(4. 6 mm x 250 mm, 5 μm) column was used in HPLC, and mobile phase was composed of acetonitrile-0.1% phosphoric acid (17:83). The detection wavelength was at 332 nm. The flow rate was 1 mL·min-1. The linear relationship of Osmundacetone was in the rang of 1.62-324 ng (r = 1.0000) and the average recovery was 99.90% (RSD = 1.67%). The contents of Osmundacetone in the Osmundae Rhizoma from different locations were 0.0267%-0.0517%.
CONCLUSIONS:
The HPLC method for determining Osmundacetone in Osmundae Rhizoma is established for the first time. It can be effectively used to controll the quality of Osmundae Rhizoma.
