Niazimicin

Pak J Pharm Sci. 2014 Mar;27(2):397-403.

Review: an exposition of medicinal preponderance of Moringa oleifera (Lank.).[Pubmed: 24577932]

METHODS AND RESULTS:
Seeds have been pragmatic with active components as novel O-ethyl-4- (α -L-rhamnosyloxy) benzyl carbamate together with seven known compounds, 4 (α -L-rhamnosyloxy)-benzyl isothiocyanate, Niazimicin, niazirin, β-sitosterol, glycerol-1- (9 -octadecanoate), 3 -O- 6 -O-oleoyl- β -D-glucopyranosyl-b-sitosterol, and β - sitosterol- 3-X-O -β -D-glucopyranoside , that have been discerned to inhibit EBV-EA (Epstein- Barr virus-early antigen), that is persuaded by the cancer promoter.
CONCLUSIONS:
M. oleifera leaves, gums, roots, flowers as well as kernels have been unanimously utilized for managing tissue tenderness, cardiovascular and liver maladies, normalize blood glucose and cholesterol. It has also profound antimicrobial, hypoglycemic and anti-tubercular activities.

J Gen Appl Microbiol. 2014;60(6):251-5.

Synergistic antimicrobial efficacy of mesoporous ZnO loaded with 4-(α-L-rhamnosyloxy)-benzyl isothiocyanate isolated from the Moringa oleifera seed.[Pubmed: 25742976 ]

The antimicrobial activities of isolated compounds from seed extracts of Moringa oleifera and synergistic antimicrobial efficacy through hybridized complex of organic-inorganic composite materials were studied.
METHODS AND RESULTS:
The two main components of the Moringa oleifera seed were isolated and determined to be Niazimicin and 4-(α-L-rhamnosyloxy)-benzyl isothiocyanate (RBI). The antimicrobial activity of the separated compounds of the Moringa oleifera seed were tested in vitro against 3 bacterial species and 2 fungal species by the paper disc diffusion assay and broth dilution methods.
CONCLUSIONS:
Both compounds showed antimicrobial activity against tested species and RBI was more effective than Niazimicin. The MIC of RBI on S. aureus, E. coli, P. aeruginosa, C. albicans, and A. niger was 0.005%, 0.1%, 0.5%, 0.5%, and 0.5%, respectively, while the MIC of Niazimicin on S. aureus was 0.1%.

Mutat Res. 1999 Apr 6;440(2):181-8.

An antitumor promoter from Moringa oleifera Lam.[Pubmed: 10209341]

METHODS AND RESULTS:
In the course of studies on the isolation of bioactive compounds from Philippine plants, the seeds of Moringa oleifera Lam. were examined and from the ethanol extract were isolated the new O-ethyl-4-(alpha-L-rhamnosyloxy)benzyl carbamate (1) together with seven known compounds, 4(alpha-L-rhamnosyloxy)-benzyl isothiocyanate (2), Niazimicin (3), niazirin (4), beta-sitosterol (5), glycerol-1-(9-octadecanoate) (6), 3-O-(6'-O-oleoyl-beta-D-glucopyranosyl)-beta-sitosterol (7), and beta-sitosterol-3-O-beta-D-glucopyranoside (8). Four of the isolates (2, 3, 7, and 8), which were obtained in relatively good yields, were tested for their potential antitumor promoting activity using an in vitro assay which tested their inhibitory effects on Epstein-Barr virus-early antigen (EBV-EA) activation in Raji cells induced by the tumor promoter, 12-O-tetradecanoyl-phorbol-13-acetate (TPA).
CONCLUSIONS:
All the tested compounds showed inhibitory activity against EBV-EA activation, with compounds 2, 3 and 8 having shown very significant activities. Based on the in vitro results, Niazimicin (3) was further subjected to in vivo test and found to have potent antitumor promoting activity in the two-stage carcinogenesis in mouse skin using 7,12-dimethylbenz(a)anthracene (DMBA) as initiator and TPA as tumor promoter. From these results, Niazimicin (3) is proposed to be a potent chemo-preventive agent in chemical carcinogenesis.

International Journal of Pharma & Bio Sciences, 2014, 5(1):B721-B729.

In silico docking studies of phytocompounds on PI3K/NF-KB mediated signalling pathway[Reference: WebLink]

Cancer cell invasion and metastasis are multistep processes influenced by the over expression of cell-secreted proteolytic enzymes such as Matrix metalloproteinases(MMPs).Phosphatidylinositide 3-kinase/Nuclear Factor of kappaB signaling pathways have been known to be involved in regulating MMP-9 expression. Synergistic targeting of these pathways using NF-B and PI3K inhibitors may have great potential for cancer treatment.
METHODS AND RESULTS:
This paper focuses on identifying phytocompounds having anticancer potential to effectively inhibit PI3K and NF-KB. Thirty five phytocompounds with anticancer properties were utilized for the study. After screening out using Lipinski rule of five and ADMET, five compounds namely allixin, capsaicin, eugenol, Niazimicin and piperine were docked with PI3K and NF-KB proteins. Niazimicin exhibited interaction for PI3K and NF-KB with residues CYS 633, ASP 632, GLN 392 and LYS 145 respectively.
CONCLUSIONS:
Niazimicin, a phytocompound of Moringa Oleifera which is an underexploited vegetable crop with medicinal properties showed maximum interaction with the targets.

Phytotherapy Research, 2010, 8(2):87-91.

Pharmacological studies on hypotensive and spasmolytic activities of pure compounds from Moringa oleifera[Reference: WebLink]

METHODS AND RESULTS:
Bioassay directed fractionation of an ethanolic extract of Moringa oleifera (MO) leaves resulted in the isolation of four pure compounds, niazinin A (1), niazinin B (2), Niazimicin (3) and niaziminin A + B (4 + 5). Intravenous administration of either one of the compounds (1–10 mg/kg) produced hypotensive and bradycardiac effects in anaesthetized rats. Pretreatment of the animals with atropine (1 mg/kg) completely abolished the hypotensive and bradycardiac effects of acetylcholine (ACh), whereas cardiovascular responses to the test compounds remained unaltered, ruling out the possible involvement of muscarinic receptor activation. In isolated guinea-pig atria all the compounds (50–150 μg/mL) produced negative inotropic and chronotropic effects. Each compound inhibited K+ -induced contractions in rabbit aorta as well as ileal contractions induced by ACh or histamine at similar concentrations. Spontaneous contractions of rat uterus were also inhibited equally by all compounds.
CONCLUSIONS:
These data indicate that the direct depressant action of these compounds exhibited on all the isolated preparations tested is probably responsible for its hypotensive and bradycardiac effects observed in vivo. Moreover, spasmolytic activity exhibited by the constituents of the plant provides a scientific basis for the traditional uses of the plant in gastrointestinal motility disorders.