Gymnemic acid I
Gymnemic acid I is a protein biosynthesis inhibitor, it as antisweet principles.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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Journal of Natural Products,2017, 80(4).
Determination of Gymnemic Acid I as a Protein Biosynthesis Inhibitor Using Chemical Proteomics.[Reference:
WebLink]
The plant Gymnema sylvestre has been used widely in traditional medicine as a remedy for several diseases, and its leaf extract is known to contain a group of bioactive triterpene saponins belonging to the gymnemic acid class.
METHODS AND RESULTS:
Gymnemic acid I (1) is one of the main components among this group of secondary metabolites and is endowed with an interesting bioactivity profile. Since there is a lack of information about its specific biological targets, the full interactome of 1 was investigated through a quantitative chemical proteomic approach, based on stable-isotope dimethyl labeling. The ribosome complex was found to be the main partner of compound 1, and a full validation of the proteomics results was achieved by orthogonal approaches.
CONCLUSIONS:
Further biochemical and biological investigations revealed an inhibitory effect of 1 on the ribosome machinery.
J Cell Physiol . 2019 Jun;234(6):9370-9377.
Gymnemic acid I triggers mechanistic target of rapamycin-mediated β cells cytoprotection through the promotion of autophagy under high glucose stress[Pubmed:
30370588]
Abstract
Gymnemic acid I (GA I) is a bioactive component of Gymnema sylvestre. It is an Indian traditional medicinal herb which has antidiabetic effect. However, the molecular mechanism is remaining to be elucidated. Here, we showed that high glucose promoted the rate of apoptosis, GA I decreased the apoptosis under the high glucose stress. Our further study explored that GA I increased the number of autophagosome and the ratio of light chain 3-I (LC3-I)/LC3-II in MIN-6 cells under the normal or high glucose stress by the methods of western blot analysis and immunofluorescence. It induced autophagy flux and inhibited the phosphorylation of mammalian target of rapamycin (mTOR) and ribosomal protein S6 kinase β-1 (p70 S6K/S6K1), which is a substrate of mTOR. GA I decreased the rate of apoptosis and the activity of caspase-3 under the high glucose stress. The inhibition of apoptosis and caspase-3 activity by GA I were increased after treating with autophagy inhibitor in mouse islet β cells MIN-6. Our data suggested that GA I-induced autophagy protected MIN-6 cells from apoptosis under high glucose stress via inhibition the phosphorylation activity of mTOR.
Keywords: apoptosis; autophagy; gymnemic acid; high glucose.
Tetrahedron Letters, 1989, 30(9):1103-1106.
Structure studies of new antisweet constituents from Gymnema sylvestre.[Reference:
WebLink]
METHODS AND RESULTS:
Gymnemic acid I , Gymnemic acid II , Gymnemic acid III and Gymnemic acid IV have been isolated from hot water extract of leaves of Gymnema sylvestre R. Br. as antisweet principles. The whole structures were determined by chemical and spectroscopic means.
