Epigoitrin

Chinese Journal of New Drugs, 2013(9).

The in vitro anti-virus effects and dose-effect relationship of epigoitrin and fructopyrano-(1→4)-glucopyranose based on [Reference: WebLink]

To investigate the in vitro anti-virus effects and dose-effect relationship of Epigoitrin and fructopyrano-(1→4)-glucopyranose(FG) from the traditional Chinese medicine Isatidis radix.
METHODS AND RESULTS:
Inhibitory effect of the target components on influenza virus neuraminidase(NA) was determined by fluorescent enzyme immunoassay.The 50% inhibitory concentration(IC50) was used as an indicator to research the relationship between Epigoitrin and FG by equivalent curve analysis.The effective concentrations of Epigoitrin and FG for in vitro anti-virus activity were determined.In the negative samples,the concentration range of target composition was gradually increased to obtain the key efficacy("incremental").Results: The equivalent point of Epigoitrin and FG samples was located below the equivalent line,and the IC50,mix value was less than IC50,add.For anti-influenza A virus activity of the incremental samples of Epigoitrin and FG,the effective concentrations were as follows: the lowest,optimum,and highest concentrations of Epigoitrin were 82.5,225.0 and 289.7 μg·mL-1;those of FG were 138.2,450.0 and 618.9 μg·mL-1.
CONCLUSIONS:
The equivalent curve analysis shows that Epigoitrin and FG exhibit cooperation.We have,for the first time,revealed the effective concentration range for in vitro anti-virus activity of Epigoitrin and FG from Isatidis radix.

J Ethnopharmacol. 2014 Nov 18;157:55-61.

In vitro antioxidant and anti-inflammatory activities of Radix Isatidis extract and bioaccessibility of six bioactive compounds after simulated gastro-intestinal digestion.[Pubmed: 25256688]

Radix Isatidis called "Ban-Lan-Gen" is one of the most commonly-used traditional Chinese medicines for antiviral, anti-inflammatory, antioxidant and antipyretic purposes. Investigate the bioaccessibility of uridine, Epigoitrin, adenosine, clemastanin B, indigoticoside A and isolariciresinol as well as the antioxidant and anti-inflammatory activities during an in vitro gastro-intestinal digestion of the Radix Isatidis extract (RIE).
METHODS AND RESULTS:
High performance liquid chromatography (HPLC) technique was adopted to determine the bioaccessibility of six bioactive compounds in RIE. Antioxidant activities of RIE in different digestive stages were determined by 1,1-Diphenyl-2-picrylhydrazyl (DPPH), superoxide anion and hydroxyl radical scavenging abilities. Anti-inflammatory activity was assayed by the inhibitions of inflammatory cytokines such as nitrous oxide (NO), prostaglandin E2 (PGE2) and tumor necrosis factor α(TNF-α) producted by lipopolysaccharide (LPS) stimulated RAW264.7 cells. The bioaccessibility of uridine, Epigoitrin, adenosine, clemastanin B, indigoticoside A and isolariciresinol were 15.38%, 18.28%, 24.01%, 6.50%, 8.65% and 17.78%, respectively. Also, the digestion products still possessed certain antioxidant activities. The antioxidant activity was highly correlated with lignans (clemastanin B, indigoticoside A and isolariciresino). The anti-inflammation activity of the three samples decreased in the order: IN sample (the solution that had diffused into the dialysis tubing)>Nondigested sample (RIE solution)>Gastric sample (post-gastric digestion)>OUT sample (material that remained in the gastro-intestinal tract).
CONCLUSIONS:
Results obtained in this research reveal the amount of bioactive compounds from RIE that could be available for absorption in vivo. The antioxidant activity decreased significantly but the anti-inflammatory activity was enhanced in serum-available fraction after gastro-intestinal digestion in vitro. This study could provide a scientific basis for a deeper pharmacological activity study of Radix Isatidis and a simple method for pharmacodynamic material basis research.

Zhongguo Zhong Yao Za Zhi. 2008 Sep;33(18):2074-6.

[Content determination of epigoitrin in Radix Isatidis and its preparation by RP-HPLC].[Pubmed: 19160787]

To establish an HPLC method for the content determination of Epigoitrin in Radix Isatidis and its preparation, and to provide valuable data for quality control of Radix Isatidis and its preparation.
METHODS AND RESULTS:
The samples were separated on a ZORBAX SB-C18 (4. 6 mm x 150 mm, 5 microm) column with the mobile phase of acetonitrile-water-phosphoric acid-triethylamine (8.50 : 90.72 : 0.73 : 0.05) in the flow rate of 0.7 mL x min(-1). The detection wavelength was set at 245 nm. Column temperature was 30 degrees C. The linear range of Epigoitrin was 0.0204-0.3060 microg (r = 0.9998), and the average recovery was 98.99% with the RSD was 1.31% (n = 9).
CONCLUSIONS:
The method for quantitation of Epigoitrin in Radix Isatidis and its preparation was accurate and reliable, which can be used to evaluate the quality of Radix Isatidis and its preparation.