Epibrassinolide
Brassinolide is a predominant plant growth modulator that regulate plant cell elongation. It is active against herpes and arena viruses.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Russian Journal of Plant Physiology, 2008, 55(2):193-200.
Effect of epibrassinolide on tyrosine phosphorylation of the calvin cycle enzymes.[Reference:
WebLink]
METHODS AND RESULTS:
The plants of Brassica juncea (L) were grown in the presence of NaCl and/or NiCl2 and were sprayed with 1muM of 24-Epibrassinolide (EBL) at 15 days after sowing (DAS) and were sampled at 30 DAS.
The plants exposed to NaCl and/or NiCl2 exhibited a significant decline in growth, the level of pigments and photosynthetic parameters. However, the follow up treatment with EBL detoxified the stress generated by NaCl and/or NiCl2 and significantly improved the above parameters. The NaCl and/or NiCl2 increased electrolyte leakage and lipid peroxidation, and decreased the membrane stability index (MSI) and relative water content. However, the EBL treatment in absence of the stress improved the MSI and relative water content but could not influence electrolyte leakage and lipid peroxidation.
CONCLUSIONS:
The antioxidative enzymes and the level of proline exhibited a significant increase in response to EBL as well as to NaCl and/or NiCl2 stress.
Planta. 1998 Dec;207(1):120-4.
Effect of epibrassinolide on hypocotyl growth of the tomato mutant diageotropica.[Pubmed:
11536896 ]
METHODS AND RESULTS:
The effect of Epibrassinolide (epiBR) on the growth and gravitropism of hypocotyls was investigated in diageotropica (dgt), a mutant of tomato (Lycopersicon esculentum Mill.). The elongation of (dgt) hypocotyls, which had been reported to be auxin-insensitive, was promoted by increasing concentrations of indole-3-acetic acid (IAA) in the presence of epiBR. alpha-(p-Chlorophenoxy)isobutyric acid, an inhibitor of auxin action, blocked the enhancement of growth by IAA and epiBR. Time course analysis of IAA-induced dgt hypocotyl elongation in the presence of epiBR revealed typical auxin response kinetics.
CONCLUSIONS:
These results suggest that epiBR restores the auxin responsiveness of dgt hypocotyls with respect to elongation. However, epiBR did not rescue the dgt phenotype with respect to shoot gravitropism. It was therefore concluded that brassino-steroid insensitivity or deficiency is not the primary defect of the dgt mutation.
