3,3'-Bilawsone
3,3'-Bilawsone is a natural product from Lomatia ferruginea.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Phytochem Anal. 2010 Sep-Oct;21(5):444-50.
Simultaneous determination of three naphthoquinones in the leaves of Impatiens balsamina L. by reversed-phase high-performance liquid chromatography.[Pubmed:
20931623 ]
Naphthoquinones; lawsone (1), lawsone methyl ether (2) and methylene-3,3'-Bilawsone (3) are the main active compounds of Impatiens balsamina leaves. To develop and validate an HPLC method for simultaneous quantitative determination of 1-3 in I. balsamina leaf extracts.
METHODS AND RESULTS:
The method utilised a Supelco® C(18) column (5 μm, 4.6 × 150 mm) at 25°C with the mixture of 2% aqueous acetic acid : methanol (gradient elution as follows: 0-10 min, 25 : 75; 10-20 min, 32 : 68; 20-35 min, 55 : 45) as the mobile phase at a flow-rate of 1 mL/min, and UV detection at 280 nm. The parameters of linearity, repeatability, reproducibility, accuracy specificity and sensitivity of the method were evaluated.
CONCLUSIONS:
The recovery of the method was 96-101% and linearity (r(2) ≥ 0.9995) was obtained for all naphthoquinones. A high degree of specificity, as well as repeatability and reproducibility (RSD less than 5%), were also achieved.
