1-Deacetylnimbolinin B
1-Deacetylnimbolinin B is a natural product from Melia toosendan Sieb. et Zucc.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Chinese Traditional & Herbal Drugs, 2015 ,46 (4) :496-501.
Chemical constituents from water extract of Toosendan Fructus by UPLC-ESI-Q-TOF-MS[Reference:
WebLink]
To analyze and identify the chemical constituents from the water extract of Toosendan Fructus by UPLC-ESIQ-TOF-MS. The analysis was performed on an ACQUITY HSS T3 reverse phase column(2.1 mm × 100 mm, 1.8 μm). The mobile phase consisted of acetonitrile and 0.1% formic acid was used for gradient elution, the flow rate was 0.4 m L/min.
METHODS AND RESULTS:
Quadrupole-time of flight-mass spectrometry was applied for the qualitative analysis under positive and negative ion modes and ESI ion source was used for mass spectra. The results indicated that fifteen compounds from the water extract of Toosendan Fructus had been identified by direct comparison in both positive and negative ion mass data, the element compositions analysis, and the data of the literature. They are vanillic acid, lilac acid, p-hydroxybenzoic acid, rutin, meliatoosenin E, toosendanin, Δ5,6-isotoosendanin, isotoosendanin, meliatoosenin N, meliatoosenin P, 1-Deacetylnimbolinin B, meliatoosenin R, 1-O-tigloyl-1-O-debenzoylohchinal, meliatoosenin R, and nimbolinin B.
CONCLUSIONS:
The efficient separation ability of UPLC and high sensitive detection of MS are used in this study, which will provide the evidences for evaluating the quality of Toosendan Fructus herbs, stabilizing the curative effect in clinic, and explaining the mechanism.
