1-Deacetylnimbolinin B

Chinese Traditional & Herbal Drugs, 2015 ,46 (4) :496-501.

Chemical constituents from water extract of Toosendan Fructus by UPLC-ESI-Q-TOF-MS[Reference: WebLink]

To analyze and identify the chemical constituents from the water extract of Toosendan Fructus by UPLC-ESIQ-TOF-MS. The analysis was performed on an ACQUITY HSS T3 reverse phase column(2.1 mm × 100 mm, 1.8 μm). The mobile phase consisted of acetonitrile and 0.1% formic acid was used for gradient elution, the flow rate was 0.4 m L/min.
METHODS AND RESULTS:
Quadrupole-time of flight-mass spectrometry was applied for the qualitative analysis under positive and negative ion modes and ESI ion source was used for mass spectra. The results indicated that fifteen compounds from the water extract of Toosendan Fructus had been identified by direct comparison in both positive and negative ion mass data, the element compositions analysis, and the data of the literature. They are vanillic acid, lilac acid, p-hydroxybenzoic acid, rutin, meliatoosenin E, toosendanin, Δ5,6-isotoosendanin, isotoosendanin, meliatoosenin N, meliatoosenin P, 1-Deacetylnimbolinin B, meliatoosenin R, 1-O-tigloyl-1-O-debenzoylohchinal, meliatoosenin R, and nimbolinin B.
CONCLUSIONS:
The efficient separation ability of UPLC and high sensitive detection of MS are used in this study, which will provide the evidences for evaluating the quality of Toosendan Fructus herbs, stabilizing the curative effect in clinic, and explaining the mechanism.