Sipeimine-3-beta-D-glucoside
Reference standards.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Chinese Journal of Experimental Traditional Medical Formulae, 2013.
Determination of Pinocembrin,Cardamonin,Sipeimine and Sipeimine-3β-D-glucoside in Houzao Niuhuangsan by HPLC-ELSD.[Reference:
WebLink]
To develop an HPLC-ELSD method for determination of the content of pinocembrin,cardamonin,sipeimine and sipeimine-3β-D-glucoside(Sipeimine-3-beta-D-glucoside) in Houzao Ninhuangsan(HZNHS).
METHODS AND RESULTS:
An Hypersil C18column was used as the chromatographic column,the flow rate was 0.9 mL·min-1.The mobile phase for pinocembrin and cardamonin consisted of methanol(A) and 0.5% phosphoric acid solution(B); the UV detection wavelength was at 300 nm.The mobile phase for sipeimine and sipeimine-3β-D-glucoside consisted of acetonitrile-water-diethylamine(65∶ 35∶ 0.5).The temperature of drift tube was set at 80 ℃,and the gas flow(N2) was set at 2.3 SLPM·min-1. There was a good linear relationship between the concentration of pinocembrin,cardamonin and peak area value when the concentrations of pinocembrin and cardamonin were within the range of 0.052 7-1.054 μg(r = 0.999 4),0.036 2-0.724 0 μg(r = 0.999 8).The average recovery were97.14%(RSD 1.10%) and 97.64%(RSD 1.39%).There was a good linear relationship when the concentrations of sipeimine and sipeimine-3β-D-glucoside were within the range of 0.057 1-1.142 μg(r = 0.9991),0.081 3-1.626 μg(r = 0.999 5).The average recovery was 97.55%(RSD 1.75%) and 98.09%(RSD1.80%).
CONCLUSIONS:
The method was accurate,sensitive,reproducible and may be used in the determination of pinocembrin,cardamonin,sipeimine and sipeimine-3β-D-glucoside in HZNHS.
