Saikosaponin H

Journal of Pharmaceutical Investigation,2008,38(1):57-61.

Determination of saikosaponin derivatives in Bupleuri Radix using HPLC-ELSD.[Reference: WebLink]

METHODS AND RESULTS:
A HPLC-ELSD method was developed to determine saikosaponin derivatives from Bupleuri Radix. Eight saikosaponins, saikosaponin c, saikosaponin i, Saikosaponin H, saikosaponin a, saikosaponin b1, saikosaponin g, saikosaponin b2, and saikosaponin d, were analyzed under optimized HPLC conditions [column: Eclipse XDB (i.d., ; mobile phase: with 0.1% COOH (v/v) for solvent A and AcCN with 0.1% COOH (v/v) for solvent B, gradient elution; flow rate: 1mL/min; injection volume: ]. Good linearity was achieved in the range from 62.5 to for each compound, and intra-day precision and accuracy at each concentration level varied between 0.05 and 5.45% and between 93.9 and 109.6%, respectively, whereas those for inter-day variations were between 0.91 to 2.73% and 94.3 to 106.1%. This HPLC-ELSD method was applied for the determination of sakosaponins from Bupleuri Radix samples, and saikosaponin a , c and d were observed as major compounds. The other saikosaponins were shown under limit of quantification level thus couldn't be quantified.
CONCLUSIONS:
The present study suggested that the introduced HPLC-ELSD method is selective and reliable, and not only saikosaponin a, but also saikosaponin c and d should be employed as the standard markers for Bupleuri Radix.