Saikosaponin H
Saikosaponin H is a natural product from Bupleurum chinense.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Determination of saikosaponin derivatives in Bupleuri Radix using HPLC-ELSD.[Reference:
WebLink]
METHODS AND RESULTS:
A HPLC-ELSD method was developed to determine saikosaponin derivatives from Bupleuri Radix. Eight saikosaponins, saikosaponin c, saikosaponin i, Saikosaponin H, saikosaponin a, saikosaponin b1, saikosaponin g, saikosaponin b2, and saikosaponin d, were analyzed under optimized HPLC conditions [column: Eclipse XDB (i.d., ; mobile phase: with 0.1% COOH (v/v) for solvent A and AcCN with 0.1% COOH (v/v) for solvent B, gradient elution; flow rate: 1mL/min; injection volume: ]. Good linearity was achieved in the range from 62.5 to for each compound, and intra-day precision and accuracy at each concentration level varied between 0.05 and 5.45% and between 93.9 and 109.6%, respectively, whereas those for inter-day variations were between 0.91 to 2.73% and 94.3 to 106.1%. This HPLC-ELSD method was applied for the determination of sakosaponins from Bupleuri Radix samples, and saikosaponin a , c and d were observed as major compounds. The other saikosaponins were shown under limit of quantification level thus couldn't be quantified.
CONCLUSIONS:
The present study suggested that the introduced HPLC-ELSD method is selective and reliable, and not only saikosaponin a, but also saikosaponin c and d should be employed as the standard markers for Bupleuri Radix.
