Methyl oleate
Methyl oleate could used as a primary carbon source and as an alternative inducer for the production of an extracellular lipase, Lip2, in Y. lipolytica strain LgX64.81 grown in a 20-l bioreactor.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Biotechnology Letters, 2005, 27(22):1751-1754.
Methyl oleate modulates LIP2 expression in the lipolytic yeast Yarrowia lipolytica.[Pubmed:
16314965]
Methyl oleate was used as a primary carbon source and as an alternative inducer for the production of an extracellular lipase, Lip2, in Y. lipolytica strain LgX64.81 grown in a 20-l bioreactor.
METHODS AND RESULTS:
The lipase-encoding gene, LIP2, was investigated during culture on Methyl oleate using a pLIP2-LacZ reporter fusion and we provide evidence for the involvement of Methyl oleate in its regulation.
The Canadian Journal of Chemical Engineering, 2016, 94(4):738-744.
Determination of adsorption and kinetic parameters for methyl oleate (biodiesel) esterification reaction catalyzed by Amberlyst 15 resin.[Reference:
WebLink]
METHODS AND RESULTS:
In this work, adsorption equilibrium constants, dispersion coefficients, and kinetic parameters were determined for the reversible esterification reaction of oleic acid with methanol, producing Methyl oleate (biodiesel) and water. The reaction was carried out at room temperature in the presence of Amberlyst 15 resin, which acts both as an adsorbent and catalyst in an HPLC column, which served as a packed bed reactor. A quasi-homogenous kinetic model coupled with a linear adsorption isotherm was explored. The elution profiles of the reactant and products were experimentally monitored and then compared with a mathematical model.
CONCLUSIONS:
The adsorption and kinetic parameters were determined by minimizing the error between the experimentally obtained elution curves and the model-predicted values using the genetic algorithm optimization technique. Further experiments were conducted under varying conditions to establish the validity of the obtained model parameters. It was also found that the system is not affected by internal and external mass transfer resistances. The mathematical model predicted the experimental outcome quite accurately.
