Jujuboside B1
Jujubosides have hypnotic effect on normal rats, may be influenced by circadian rhythm and the serotonergic system may involve in the hypnotic effect of jujubosides, jujubosides may be good source of lead compounds for novel hypnotics.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Analytical Methods, 2014, 6.
Analysis of six bioactive components in Semen Ziziphi Spinosae by UPLC-ELSD and UPLC-Q/TOF-MS.[Reference:
WebLink]
METHODS AND RESULTS:
A simple and sensitive method was first developed for the simultaneous determination of six bioactive compounds in Semen Ziziphi Spinosae, which is commonly used in traditional Chinese medicines, by ultra-performance liquid chromatography coupled with an evaporative light scattering detector (UPLC-ELSD). Furthermore, the main compounds were identified using UPLC coupled with electrospray ionization and time-of-flight mass spectrometry (ESI-Q/TOF-MS). The separation of the compounds of interest was performed on a BEH C18 column with acetonitrile and water (0.1% aqueous formic acid) as the mobile phase. Six analytes (spinosin, jujuboside A, jujuboside D, jujuboside B, Jujuboside B1, betulinic acid) demonstrated good linearity (r2 > 0.9984) over a relatively wide concentration range. The method revealed high average recovery (range, 94.36-99.49%) and good precision with inter- and intra-day variations with RSD less than 4.72%. The limits of detection (LOD) ranged from 10.4-31.2 ng, while the limits of quantification (LOQ) were defined in the range of 21.9-84.0 ng. The validated method was successfully applied to quantitatively analyze 28 samples of different places from China. The results show there are great variations among the contents of the six ingredients.
CONCLUSIONS:
These results demonstrate that this approach has the potential for the quality control of Semen Ziziphi Spinosae.
