Frangulin B

Frangulin B
Product Name Frangulin B
CAS No.: 14101-04-3
Catalog No.: CFN70270
Molecular Formula: C20H18O9
Molecular Weight: 402.4 g/mol
Purity: >=98%
Type of Compound: Anthraquinones
Physical Desc.: Powder
Source: The cortex of Rhamnus frangula L.
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
Price:
Reference standards.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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    Validated method for the analysis of frangulins A and B and glucofrangulins A and B using HPLC and UHPLC.[Reference: WebLink]
    In the present study, robust and validated HPLC and UHPLC methods for the quantitative determination of frangulin A and Frangulin B (3 and 4) and glucofrangulins A and B (1 and 2) in the bark of Frangula alnus have been developed.
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    The HPLC method allowed the separation of the analytes in 25 min and the UHPLC method in just 13 min. The HPLC method used an MN Nucleodur C18 125 × 4 mm column with 3 μm particles, while the UHPLC method used a Waters Acquity UPLC BEH C18, 100 × 2.1 mm column with 1.7 μm particles. Mobile phase A consisted of water and 1.25 mL/L phosphoric acid (85%), while mobile phase B consisted of CH3CN/MeOH (20:80). The flow rates were set to 1 mL/min for the HPLC method and 0.4 mL/min for the UHPLC method, with the column temperature held at 50 °C and the detection wavelength being 435 nm for either method. A fractional factorial design was used to check the robustness of the methods.
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