Bulleyanin
Bulleyanin shows strong inhibition of mouse ehrlich ascites carcinoma (ECA), mouse sarcoma S-180, and mouse liver ascites.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Acta Botanica Yunnanica.,1985, 8(4):79-84.
THE CHEMICAL STRUCTURE OF BULLEYANIN[Reference:
WebLink]
METHODS AND RESULTS:
During the course of our studies on the biologically active constituents of Rabdosia plants, we examined the etrereal extract of the leaves of Rcbdosia bulleyana (Diels) Hara collected in the Cangshan Mountain, Yunnan, China. A new diterpcnoid having a ent-Kaurene-skelcton, Bulleyanin, was isolated. Its yield is 0.4% of the leaves and it shows tumor-inhibitor activities for S-180, ECA, and etc. of mice. The structure of the new compound was elucidated by spectroscopic and chemical evidences as follows; Bulleyanin, mp 240-244℃, C28 H38 O10, ent-12β-hydroxy-lα, 3α, 7β, 14α-tetraacetoxy-16-Kauren-15-one ( I ).
CONCLUSIONS:
1H XMR data of Bulleyanin and its derivatives, 13C NMR data of Bulleyanin and dehydrobulleynnin have been assigned. 13C NMR assignments are based on a combination of proton noise decoupling, off-resonance decoupling and comparison with related compounds.
