Anisatin
Anisatin, a toxic, insecticidally active component of Sikimi plant, is a potent GABA antagonist.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
Eur J Pharmacol.2024, 978:176800.
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Food Funct.2022, D1FO03838A.
Dent Mater J. 2024, dmj.2023-294.
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Neuroscience Letters, 1981, 25(1):83-88.
Anisatin, a potent GABA antagonist, isolated from Illicium anisatum.[Reference:
WebLink]
METHODS AND RESULTS:
The neuropharmacological properties of Anisatin were tested on the frog spinal cord and the crude synaptic membrane from rat brain. Anisatin (10−5 M) reduced the amplitude of dorsal root potentials induced by stimulation of the adjacent dorsal root and presynaptic inhibition of the ventral root reflex. Anisatin shifted the dose-response curve for GABA-induced depolarization in the primary afferent terminal to the right and also reduced the maximum response to GABA. [3H]Muscimol binding to the crude synaptic membrane was not inhibited by Anisatin.
CONCLUSIONS:
These results indicate that Anisatin is a picrotoxin-like, non-competititve GABA-antagonist.
British journal of pharmacology, 1999,127(7):1567-1576.
Anisatin modulation of the gamma-aminobutyric acid receptor-channel in rat dorsal root ganglion neurons.[Reference:
WebLink]
1. Anisatin, a toxic, insecticidally active component of Sikimi plant, is known to act on the GABA system. In order to elucidate the mechanism of Anisatin interaction with the GABA system, whole-cell and single-channel patch clamp experiments were performed with rat dorsal root ganglion neurons in primary culture.
METHODS AND RESULTS:
2. Repeated co-applications of GABA and Anisatin suppressed GABA-induced whole-cell currents with an EC50 of 1.10 microM. No recovery of currents was observed after washout with Anisatin-free solution. 3. However, pre-application of Anisatin through the bath had no effect on GABA-induced currents. The decay phase of currents was accelerated by Anisatin. These results indicate that Anisatin suppression of GABA-induced currents requires opening of the channels and is use-dependent. 4. Anisatin suppression of GABA-induced currents was not voltage dependent. 5. Picrotoxinin attenuated Anisatin suppression of GABA-induced currents. [3H]-EBOB binding to rat brain membranes was competitively inhibited by Anisatin.
CONCLUSIONS:
These data indicated that Anisatin bound to the picrotoxinin site. 6. At the single-channel level, Anisatin did not alter the open time but prolonged the closed time. The burst duration was reduced and channel openings per burst were decreased indicating that Anisatin decreased the probability of openings.
