4'-Methoxyagarotetrol
Reference standards.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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Chinese Traditional Patent Medicine, 2011.
Preparative isolation and purification of agarotetrol and 4′-methoxyagarotetrol from Aquilaria sinensis (Lour.) Gilg by high-speed counter-current chromatography[Reference:
WebLink]
To establish the method of separating 2-(2-Phenylethyl)chromone derivatives from agalwood by high-speed counter-current chromatography(HSCCC).
METHODS AND RESULTS:
Chloroform-methanol-water(4:2.6:2.4,v/v/v) was used as the two-phase solvent system.The upper phase was used as the stationary phase,the revolution speed was 900 rpm and the flow rate was 1.2 mL/min. A preparative HSCCC method was successfully established for separating agarotetrol and 4′-methoxyagarotetrol from 95% ethanol extract of Aquilaria sinensis(Lour.)Gilg.Identification of two constituents was carried out according to its EI-MS、1H-NMR and 13C-NMR da-ta.The purity of agarotetrol and 4′-methoxyagarotetrol was 99.0%,as determined by HPLC.
CONCLUSIONS:
HSCCC is successfully used for the separation and purification of two main 2-(2-Phenylethyl)chromone derivatives which has similar structure from agalwood with ideal purity.
