2-O-Acetyl-20-hydroxyecdysone
Reference standards.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Chemical constituents from roots tubers of Serratula chinensis[Reference:
WebLink]
To study the chemical constituents from the roots tubers of Serratula chinensis.
METHODS AND RESULTS:
The chemical constituents were separated and purified by silica gel, Sephadex LH-20, ODS column chromatographies, and preparative HPLC. Their structures were determined by physicochemical properties and spectral data. Sixteen compounds(ecdysteroids) were isolated from the n-butanol fraction of 95% ethanol extract in the root tubers of S. chinensis, and their structures were identified as 20-hydroxyecdysone(1), polypodine B(2), carthamosterone(3), 20-hydroxyecdysone-20, 22-monoacetonide(4), 24-epi-abutasterone(5), polypodine C(6), coronatasterone(7), 20-hydroxyecdysone 2-O-β-D-glucopyranoside(8), 20-hydroxyecdysone 25-O-β-Dglucopyranoside(9), polypodine B 20, 22-acetonide(10), shidasterone(11), 2-O-Acetyl-20-hydroxyecdysone(12), 3-O-acetyl-20-hydroxyecdysone(13), 20-hyderoxyecdysone-20, 22-butylidene acetal(14), 24-methylene-shidasterone(15), and ajugasterone D(16).
CONCLUSIONS:
Compounds 2, 4, 5, 7—10, 12, 15, and 16 are isolated from this plant for the first time, and compounds 5, 7—10, and 16 are found in the plants of genus Serratula L. for the first time.
