14-Dehydrobrowniine
Reference standards.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Chemical & Pharmaceutical Bulletin, 2009, 35(6):2615-2617.
STRUCTURES OF TOROKONINE AND GOMANDONINE, TWO NEW DITERPENE ALKALOIDS FROM ACONITUM SUBCUNEATUM NAKAI[Reference:
WebLink]
METHODS AND RESULTS:
Chemical investigation of the roots of Aconitum subcuneatum Nakai resulted in the isolation of two new diterpene alkaloids torokonine (1) and gomandonine (2), along with eight known compounds, i.e. mesaconitine, jesaconitine, 14-Dehydrobrowniine, neoline, 15-α-hydroxyneoline, isotalatizidine, senbusine A, and virescenine. The structure and the absolute configuration of torokonine (1) were established as 7-α-hydroxy ryosenamine from the spectroscopic analysis.
CONCLUSIONS:
The novel structure of gomandonine (2), having an epoxy ring, was initially deduced by the pyridine-induced solvent shift technique and then confirmed by X-ray analysis.
