Wilfortrine
Wilfortrine can induce liver cancer cell HepG2 apoptosis, but with no effect on the cell cycle, mainly by promoting Bax expression and inhibiting anti-apoptotic protein Bcl-2 expression. The combined treatment using wilfortrine and paclitaxel can inhibit proliferation and invasion of liver cancer cells via down-regulating Bcl-2 and up-regulating Bax, with better efficacy than single use of either drug. Wilfortrine and euonine (40.80 mg/(kg.d) x 4d ip) show marked depressant effects on humoral mediated immunity using the hemolysin reaction as indices.
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Genet Mol Res. 2015 Nov 30;14(4):15349-55.
Effect of wilfortrine on human hepatic cancer HepG2 cell proliferation potential in vitro.[Pubmed:
26634500 ]
Liver cancers are characterized by high morbidity and mortality owing to few effective drugs for its treatment. Wilfortrine has several pharmacological effects, including an inhibitory effect on liver cancer cell proliferation. However, whether Wilfortrine can induce liver cancer cell apoptosis has not been elucidated.
METHODS AND RESULTS:
We investigated the role of Wilfortrine on liver cancer cell HepG2 apoptosis and analyzed its possible mechanisms to provide a theoretical basis for clinical analysis of liver cancer pathogenesis. The liver cancer cell line HepG2 was treated with 40 mM Wilfortrine for 48 h. Flow cytometry was applied to detect HepG2 cell apoptosis and cell cycle changes. Western blot was used to analyze Bcl-2 and Bax expression. The HepG2 cell apoptosis rate increased significantly after treatment with Wilfortrine, especially the early apoptosis rate (P < 0.05). However, Wilfortrine did not change the cell cycle of HepG2 cells. After Wilfortrine treatment, Bcl- 2 expression decreased significantly (P < 0.05); on the contrary, Bax expression increased noticeably compared with the control group (P < 0.05).
CONCLUSIONS:
Wilfortrine can induce liver cancer cell HepG2 apoptosis, but with no effect on the cell cycle, mainly by promoting Bax expression and inhibiting anti-apoptotic protein Bcl-2 expression.
Yao Xue Xue Bao. 1989;24(8):568-72.
Immunosuppresive effects of wilfortrine and euonine.[Pubmed:
2618700]
Wifortrine and Euonine isolated from Tripterygium wilfordii Hook. f.
METHODS AND RESULTS:
Wilfortrine and euonine (40.80 mg/(kg.d) x 4d ip) showed marked depressant effects on humoral mediated immunity using the hemolysin reaction as indices. Wilfortrine (160 mg/kg.d x 9d ip) exhibited a depressant effect on graft vs host reaction (GVHR), but Wilfortrine (80 mg/(kg.d) x 9d ip) showed no definite effect. Euonine (80 mg/(kg.d) x 10d ip) showed marked suppressant effects on DNCB induced delayed hypersensitivity reaction on skin in mice. Wilfortrine and Euonine (80 mg/(kg.d) x 4d ip) significantly decreased the clearance rate of charcoal particles and the weights of spleen and thymus.
Med Sci Monit. 2016 Apr 4;22:1109-14.
Effect of Combined Treatment Using Wilfortrine and Paclitaxel in Liver Cancer and Related Mechanism.[Pubmed:
27043783 ]
Liver cancer is a common malignant tumor with high mortality. Currently, effective medicines against liver cancer are still lacking. Paclitaxel is a wide-spectrum anti-tumor agent, while Wilfortrine has been shown to have an inhibitory effect on the proliferation of liver cancer cells. This study thus investigated the potential effect of paclitaxel combined with Wilfortrine on cultured liver cancer cells and related mechanisms, in order to provide evidence for pathogenesis and treatment of liver cancer.
METHODS AND RESULTS:
Liver cancer cell line HpeG2 was divided into control, paclitaxel, Wilfortrine, and combined treatment groups. Cell proliferation was tested by MTT, while invasion was detected in Transwell chamber assay. Apoptotic protein Bcl-2 and Bax expression levels were further quantified using real-time PCR and Western blotting.
Both of those 2 drugs can effectively inhibit cancer cell proliferation, depress invasion ability, increase Bcl-2 expression, and elevate Bax expression levels (p<0.05 in all cases). The combined therapy had better treatment efficacy compared to either of those drugs alone (p<0.05).
CONCLUSIONS:
The combined treatment using Wilfortrine and paclitaxel can inhibit proliferation and invasion of liver cancer cells via down-regulating Bcl-2 and up-regulating Bax, with better efficacy than single use of either drug.
