N-Nornuciferine
N-Nornuciferine can competitively inhibit CYP2D6 activity with Ki values of 2.34 uM.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Microchemical Journal2024: 196:109676.
Acta Chromatographica2021, 00960.
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Food Bioscience2023, 52:102412
Sci Rep. 2018, 1-9
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Toxicol Appl Pharmacol.2021, 427:115668.
Braz J Med Biol Res.2021, 54(12):e11183.
J. Traditional Thai Medical Res. 2022,8(1):1-14.
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J Ethnopharmacol. 2014 Apr 11;153(1):190-6.
Identification and characterization of potent CYP2D6 inhibitors in lotus leaves.[Pubmed:
24561383]
METHODS AND RESULTS:
Five probe substrates were incubated with human liver microsomes in the presence or absence of the LAE, the alkaloid fraction (AF), the flavonoid fraction (FF), or the individual aporphine alkaloids, namely, nuciferine (NF), N-Nornuciferine (N-NF), and 2-hydroxy-1-methoxyaporphine (HMA).
After the incubation, the relative metabolites of the substrates were analyzed using LC-MS/MS. The results showed that the LAE strongly inhibited CYP2D6 with an IC50 value of 12.05µg/mL and weakly inhibited other isoenzymes. In addition, FF was found to weakly inhibit CYP2D6, whereas AF exerted a markedly higher inhibitory effect on CYP2D6 activity with an IC50 value of 0.96µg/mL. The three aporphine alkaloids isolated from the AF (NF, N-Nornuciferine, and HMA) significantly inhibited CYP2D6 with IC50 values of 3.78, 3.76, and 3.15µM, respectively.
CONCLUSIONS:
Their Lineweaver-Burk plots and Dixon plots showed that NF, N-Nornuciferine, and HMA competitively inhibited CYP2D6 activity with Ki values of 1.88, 2.34, and 1.56µM, respectively.
Talanta. 2010 Jan 15;80(3):1292-7.
Application of ionic liquids based microwave-assisted extraction of three alkaloids N-nornuciferine, O-nornuciferine, and nuciferine from lotus leaf.[Pubmed:
20006090]
The application of ionic liquids based microwave-assisted extraction (ILMAE) was successfully developed for extracting three alkaloids N-Nornuciferine, O-nornuciferine, and nuciferine from lotus leaf.
METHODS AND RESULTS:
Seven kinds of 1-alkyl-3-methylimidazolium with different cations and anions were investigated in this work and 1.0M 1-hexyl-3-methylimidazolium bromide ([C(6)MIM]Br) solution was selected as solvent. In addition, the microwave parameters including irradiation power, extraction time and solid-liquid ratio were optimized. Compared with the regular MAE and conventional heat-reflux extraction (HRE), the proposed approach exhibited higher efficiency (0.9-43.7% enhanced) and shorter extraction time (from 2h to 2min), which indicated ILMAE was an efficient, rapid and simple sample preparation technique. Moreover, the proposed method was validated by the linearity, reproducibility, and recovery experiments. Good linearity was observed with the regression coefficients (r(2)) between 0.9998 and 0.9999.
CONCLUSIONS:
The recoveries of all methods were in the range of 94.6% and 105.5% with RSD lower than 6.6%, which indicated that the proposed method was credible.
