(-)-Licarin B
Licarin B can improve insulin sensitivity via PPARγ and activation of GLUT4 in the IRS-1/PI3K/AKT pathway in 3T3-L1 adipocytes, it as a promising bioactive for insulin resistance and associated complications through its partial PPARγ activity.
Inquire / Order:
manager@chemfaces.com
Technical Inquiries:
service@chemfaces.com
Tel:
+86-27-84237783
Fax:
+86-27-84254680
Address:
1 Building, No. 83, CheCheng Rd., Wuhan Economic and Technological Development Zone, Wuhan, Hubei 430056, PRC
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
Food Chem.2023, 424:136383.
ACS Omega.2022, 7(44):40009-40020.
Int J Vitam Nutr Res.2022, doi: 10.1024.
Drug Chem Toxicol.2024, 1-10.
BMC Microbiol.2019, 19(1):78
J Nat Med.2020, 74(1):65-75
Pharmacognosy Journal2019, 11,6:1235-1241
J Ethnopharmacol.2024, 320:117426.
J Sep Sci.2019, 42(21):3352-3362
Int J Mol Sci.2022, 23(24):16000.
Related and Featured Products
Rsc Adv., 2016, 6(83):79859-70.
Licarin B from Myristica fragrans improves insulin sensitivity via PPARγ and activation of GLUT4 in IRS-1/PI3K/AKT pathway in 3T3- L1 adipocytes[Reference:
WebLink]
Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors regulating lipid and glucose metabolism.
METHODS AND RESULTS:
The objective of the present study is to characterize the cellular effect of Licarin B (LB), a neolignan isolated from Myristica fragrans on the PPARγ and insulin signaling pathways in 3T3-L1 preadipocytes. The molecular mechanism of action of LB on PPARγ and insulin signaling pathways were studied using in vitro and in silico methods. Functional activation of PPARγ in vitro was confirmed by 3T3-L1 preadipocyte differentiation, regulation of target genes and protein expression. LB caused triglyceride accumulation during adipogenesis but significantly less compared to rosiglitazone (RG), a PPARγ full agonist. In in vitro time-resolved fluorescence resonance energy transfer-based competitive binding assay, LB showed an IC50 value of 2.4 μM whereas for RG and GW9662 it was 57.96 nM and 18.68 nM respectively. Virtual screening of LB with PPARγ showed hydrophobic interactions with a binding energy of -9.36 kcal mol⁻1. Interestingly enough LB improved insulin sensitivity by up regulating the GLUT4 expression and translocation via IRS-1/PI3K/AKT pathway, enhanced adiponectin secretion and modulated mRNA expression profile of PPARγ target genes C/EBPα, IRS-2, and LPL significantly compared to RG.
CONCLUSIONS:
This scientifically validate LB as a promising bioactive for insulin resistance and associated complications through its partial PPARγ activity.
