Kaempferol-3-beta-O-glucuronide

Z Naturforsch C. 2007 Sep-Oct;62(9-10):656-60.

Evaluation of cytotoxic compounds from calligonum comosum L. growing in Egypt.[Pubmed: 18069236]

METHODS AND RESULTS:
Calligonum comosum (Polygonaceae), an Egyptian desert plant, was extracted and fractionated using petroleum ether, methylene chloride, and ethyl acetate. The total methanolic extract and other fractions were tested for their anticancer activity using Ehrlich ascites, brine shrimp and antioxidant assays. Ethyl acetate fraction proved to be the most active in all assays. Eight compounds were isolated, purified, and identified from this fraction as (+)-catechin (1), dehydrodicatechin A (2), kaempferol-3-O-rhamnopyranoside (3), quercitrin (quercetin-3-O-rhamnopyranoside) (4), beta-sitosterol-3-O-glucoside (5), isoquercitrin (quercetin-3-O-glucopyranoside) (6), kaempferol-3-O-glucuronide (Kaempferol-3-beta-O-glucuronide,7), and mequilianin (quercetin-3-O-glucuronide) (8).
CONCLUSIONS:
All isolated compounds were tested for their cytotoxicity and antioxidant activity. Compound 2 showed the best cytotoxic and antioxidant activity.

Int Immunopharmacol . 2018 Apr;57:62-71.

Kaempferol-3-o-β-d-glucuronate exhibit potential anti-inflammatory effect in LPS stimulated RAW 264.7 cells and mice model[Pubmed: 29475097]

Abstract Kaempferol-3-O-β-d-glucuronide (K3G) having various pharmacological effects was explored for its anti-inflammatory effect in LPS induced RAW 264.7 cells and mice model. K3G significantly inhibited various pro-inflammatory mediators like IL-1β, NO, PGE2, and LTB4. It upregulated the secretion of anti-inflammatory cytokine IL-10. K3G is found to reduce inflammation when studied for parameters like phagocytic index, carrageenan induced paw edema in mice and organ weight. It reduced inflammation in a dose dependent manner both in-vitro and in-vivo. Further molecular insights into the study reveal that K3G blocks the phosphorylation of NF-kB which is key regulator of inflammation, thereby exhibiting anti-inflammatory potential. Hence, this study permits further investigation to develop K3G as anti-inflammatory drug. Keywords: Anti-inflammatory; Histopathology; Pathway elucidation; Proinflammatory.