Homopahutoxin
Homopahutoxin is a haemolytic agent.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Int J Mol Sci.2019, 20(16):E4015
Heliyon.2024, 10(11):e32352.
Food Funct.2020, 11(2):1322-1333.
Clin Transl Oncol.2019, 10.1007
J Ethnopharmacol.2022, 289:115018.
J Mol Med (Berl).2018, 96(7):661-672
Anal Biochem.2019, 569:10-15
Chemistry of Natural Compounds2018, 54(3):572-576
Sci Rep.2024, 14(1):26330.
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Toxicon. 1987;25(4):459-61.
Occurrence of pahutoxin and homopahutoxin in the mucus secretion of the Japanese boxfish.[Pubmed:
3617085]
METHODS AND RESULTS:
The mucus secreted by the Japanese boxfish O. immaculatus was extracted with n-butanol. The extract was fractionated by silica gel open column chromatography and reversed phase high performance liquid chromatography to give two hemolytic components.
CONCLUSIONS:
The major hemolysin was identical with pahutoxin which was previously isolated from the Hawaiian boxfish O. lentiginosus, while the other hemolysin was a new choline chloride ester, named Homopahutoxin.
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