Diosbulbin D
Diosbulbin D shows direct toxic effect on hepatocytes, the mechanism could be associated with oxidative stress.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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Toxicity of a Diterpene Lactone Isolated from Dioscorea bulbifera on Hepatocytes[Reference:
WebLink]
To test the toxic effects of a diterpene lactone, diosbulbin-D, isolated from Dioscorea bulbifera L. on hepatocytes.
METHODS AND RESULTS:
Using the MTT assay to test the effect of Diosbulbin D on hepatocytes.Selected intracellular enzymes release levels by diosbulbin-D were determined to further validate the toxic effect on hepatocytes.Furthermore,the level of intracellular glutathione and the induction of reactive oxygen species(ROS) by diosbulbin-D were measured by fluorometric and flow cytometry methods. Diosbulbin-D showed toxic effect on hepatocytes and a significant increase in intracellular release levels of LDH and the liver enzymes,alanine aminotransferase and aspartate aminotransferase confirmed diosbulbin-D's toxic effect.ROS increase was related to the decrease in level of GSH.Cell pretreated NAC almost completely blocked the ROS fluorescence and recovered the cell growth inhibition induced by diosbulbin-D.
CONCLUSIONS:
Diosbulbin-D shows direct toxic effect on hepatocytes.The mechanism could be associated with oxidative stress.
Phytochemistry.1984;23(3):623–625.
Diosbulbin d and 8-epidiosbulbin e acetate, norclerodane diterpenoids from dioscorea bulbifera tubers.[Reference:
WebLink]
METHODS AND RESULTS:
From tubers of Dioscorea buibifera L. var sativa a new 18-norclerodane diterpenoid, 8-epidiosbulbin E acetate, has been isolated, besides the previously known norditerpenoid, Diosbulbin D, and its structure established by spectroscopic means.
