13-Oxo-9E,11E-octadecadienoic acid | CAS:29623-29-8

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Journal of Chromatography A, 2017, 1487:162-167.

Screening and analysis of potential anti-tumor components from the stipe of Ganoderma sinense using high-performance liquid chromatography/time-of-flight mass spectrometry with multivariate statistical tool.[Reference: WebLink]

According to Chinese Pharmacopoeia 2015 edition, Ganoderma (Lingzhi) is a species complex that comprise of Ganoderma lucidum and Ganoderma sinense. The bioactivity and chemical composition of G. lucidium had been studied extensively, and it was shown to possess antitumor activities in pharmacological studies. In contrast, G. sinense has not been studied in great detail. Our previous studies found that the stipe of G. sinense exhibited more potent antitumor activity than the pileus.
METHODS AND RESULTS:
To identify the antitumor compounds in the stipe of G. sinense, we studied its chemical components by merging the bioactivity results with liquid chromatography-mass spectrometry-based chemometrics.The stipe of G. sinense was extracted with water, followed by ethanol precipitation and liquid–liquid partition. The resulting residue was fractionated using column chromatography. The antitumor activity of these fractions were analysed using MTT assay in murine breast tumor 4T1 cells, and their chemical components were studied using the LC-QTOF-MS with multivariate statistical tools. The chemometric and MS/MS analysis correlated bioactivity with five known cytotoxic compounds, 4-hyroxyphenylacetate, 9-oxo-(10E,12E)-octadecadienoic acid, 3-phenyl-2-propenoic acid, 13-Oxo-9E,11E-octadecadienoic acid and lingzhine C, from the stipe of G. sinense.
CONCLUSIONS:
To the best of our knowledge, 4-hyroxyphenylacetate, 3-phenyl-2-propenoic acid and lingzhine C are firstly reported to be found in G. sinense. These five compounds will be investigated for their antitumor activities in the future.

Product Name	13-Oxo-9E,11E-octadecadienoic acid
CAS No.:	29623-29-8
Catalog No.:	CFN80102
Molecular Formula:	C₁₈H₃₀O₃
Molecular Weight:	294.43 g/mol
Purity:	>=98%
Type of Compound:	Lipids
Physical Desc.:	Powder
Source:	The leaves of Artemisia argyi
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Price:	$368/5mg